Freeze-drying of Aegyptianella pullorum.

نویسندگان

  • R A Bartkowiak
  • F W Huchzermeyer
  • F T Potgieter
  • L van Rensburg
  • F J Labuschagne
  • B J van Biljon
چکیده

Heparinized whole blood, parasitized with Aegyptianella pullorum, was collected from 2 fowls. Buffered lactose peptone (BLP) was added v/v as a stabilizer and the mixture lyophilized in 2 ml aliquots after rapid or slow freezing. At different stages during the freeze-drying process, as well as after lyophilization and reconstitution with 1.8 ml of sterile water, samples were taken and injected into pullets. Infectivity was maintained throughout. However, the prepatent period was lengthened after freezing and particularly after lyophilization when there was some loss of viability.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Aegyptianella-Like organisms and microfilariae in a severely diseased Bittern (Botaurus stellaris stellaris)

Blood parasites are often found in the blood smears of domestic and wild-caught birds [12,14]. Of these, a member of the family Anaplasmataceae, Aegyptianella (CARPANO, 1929) genus has been reported from domestic fowl, ducks, geese, turkeys, pigeons, quails, ostrichs and psittacine birds [9, 13,14]. The only named species is Aegyptianella pullorum, known in the past as Babesia pullorum or Balfo...

متن کامل

Description of Aegyptianella botuliformis n. sp. (Rickettsiales: Anaplasmataceae) from the helmeted guineafowl, Numida meleagris.

Aegyptianella botuliformis n. sp. (Rickettsiales: Anaplasmataceae) isolated from helmeted guineafowls Numida meleagris from the Kruger National Park is described. The rickettsia occurs within a membrane-bound vacuole in the cytoplasm of erythrocytes with up to 8 organisms in a mature inclusion. The initial body resembles that of Aegyptianella pullorum. The tightly packed, sausage-shaped interme...

متن کامل

Modeling and Optimization of Ultrasound-Assisted Osmotic Dehydration with Finished Freeze Drying on Black Cherries – The Effect on Antioxidant Activities

ABSTRACT: In this study, response surface methodology was used to optimize ultrasound osmotic pretreatment with finished freeze drying of black cherries. Freeze drying is a separation process based on the  sublimation phenomenon. This process has the following advantages as compared to the conventional drying process, maintenance of the structure, moisture removal at low temperature (reduced tr...

متن کامل

Sperm freeze-drying; effects on sperm parameters

Today, sperm cryopreservation is a routine practice in infertility clinics. One of the alternative methods to preserve sperm is freeze-drying. This process consists of three steps. The first step is sample freezing, during which the solvent is separated from the solvent. Water forms ice crystals and the solute is placed between the ice crystals. Freezing may be done in a separate device or in a...

متن کامل

Avian pulmonary hypertension syndrome. IV. Increased right ventricular mass in turkeys experimentally infected with Plasmodium durae.

Infection of young turkeys with Plasmodium durae produced a significant increase in the mean relative right ventricular mass from 0.172 in the controls to 0.208 in the infected group. Although turkeys react less strongly to hypoxia than broiler chickens, the similarity of this finding to that previously reported in broilers infected with Aegyptianella pullorum suggests that the hypoxic effect o...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Onderstepoort journal of veterinary research

دوره 55 2  شماره 

صفحات  -

تاریخ انتشار 1988